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The effect of freeze-storage on culturable soil microorganism viability was determinedfor soil samples from three agricultural maize crop systems (under conventional management,direct seeding, and direct seeding with a cover crop). Most culturable soil bacteria wereunaffected by the freeze-thaw stress or the prolonged freeze-storage, but the viability of moldand Bacillus mycoides populations decreased drastically after eight months of freeze-storage, limitingthe process sustainability to this period. However, these changes did not significantly affect either thetotal microorganism biodiversity index or the biodiversity differences between treatment. Based onthe available results, freeze-storage seems to be a sustainable process for up to eight months that canbe allowed in analyses of culturable microbial population biodiversityinfo:eu-repo/semantics/publishedVersio

Evaluation of the Microbial Viability of Soil Samples from Maize Crops in Freeze-Storage under Different Management Conditions in a Semi-Arid Climate