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For a holistic evaluation of sustainability, the economic and environmental aspects should be considered jointly to avoid trade-offs between the two dimensions. In this manuscript, the themes addressed, and the approaches used in this Special Issue “Economic and Environmental Impact Assessment of Renewable Energy from Biomass” to investigate the sustainability are summarized. Different approaches such as Energy Analysis, Life Cycle Assessment, technical and economic evaluation of key processes are applied to different renewable energy pathways (biogas, wood biomass, by-product valorization, etc.). The different manuscripts accepted in this Special Issue increases our comprehension and understanding of the relation between economic and environmental performances of renewable energy from biomass.