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Soil carbon and nitrogen storage and stabilization are the key to solving the problems of mitigation of global warming and maintaining of crop productivity. In this study, the contents of soil organic carbon (SOC) and soil organic nitrogen (SON) and their stable isotope compositions (δ13CSOC and δ15NSON) in soil profiles were determined in two agricultural lands (including a farmland and an abandoned farmland) and four non-agricultural lands (including two shrub-grass lands and two shrub lands) in the karst critical zone observatory (CZO), Southwest China. The contents of SOC and SON were used for research on the effects of land use on SOC and SON storage, and the change of δ13CSOC and δ15NSON values in soil profiles were used to indicate SOC and SON stabilization. The results showed that agricultural activities reduced SOC and SON storage in the whole soil layers of farmland compared to non-agricultural lands, and farmland abandonment slightly increased SOC and SON storage. Crop rotation between peanut (C3) and corn (C4) affected the δ13CSOC in surface soils of agricultural lands (−21.6‰), which were intermediate between shrub lands (−22.7‰) and shrub-grass lands (−19.6‰). 15N-depleted SON in surface soils in farmland compared to those soil in other lands possibly associated with synthetic N fertilizer application. In soil layers below 30 cm depth the δ13CSOC deceased with depth, while the δ15NSON displayed irregular fluctuation. The change in δ13CSOC and δ15NSON through soil profiles in karst soils were more intensive than those in semiarid grassland soils indicating the less stabilization of SOC and SON in karst soils.

Variations and Indications of δ13CSOC and δ15NSON in Soil Profiles in Karst Critical Zone Observatory (CZO), Southwest China