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An amperometric biosensor for hydrogen peroxide (H2O2) was developed viaan easy and effective enzyme immobilization method with the Ã¢Â€ÂœsandwichÃ¢Â€Â configuration:ferrocene-chitosan: HRP: chitosan-glyoxal using a glassy carbon electrode as the basicelectrode. In order to prevent the loss of immobilized HRP under optimized conditions,the biosensor surface was cross-linked with glyoxal. Ferrocene was selected andimmobilized on the glassy carbon electrode surface as a mediator. The fabricationprocedure was systematically optimized to improve the biosensor performance. Thebiosensor had a fast response of less than 10 s to H2O2, with a linear range of 3.5ÃƒÂ—10-5 to1.1ÃƒÂ—10-3 M, and a detection limit of 8.0ÃƒÂ—10-6 M based on S/N = 3

sensors

A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide