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Transmission multispectral imaging (TMI) has potential value for medical applications, such as early screening for breast cancer. However, because biological tissue has strong scattering and absorption characteristics, the heterogeneity detection capability of TMI is poor. Many techniques, such as frame accumulation and shape function signal modulation/demodulation techniques, can improve detection accuracy. In this work, we develop a heterogeneity detection method by combining the contour features and spectral features of TMI. Firstly, the acquisition experiment of the phantom multispectral images was designed. Secondly, the signal-to-noise ratio (SNR) and grayscale level were improved by combining frame accumulation with shape function signal modulation and demodulation techniques. Then, an image exponential downsampling pyramid and Laplace operator were used to roughly extract and fuse the contours of all heterogeneities in images produced by a variety of wavelengths. Finally, we used the hypothesis of invariant parameters to do heterogeneity classification. Experimental results show that these invariant parameters can effectively distinguish heterogeneities with various thicknesses. Moreover, this method may provide a reference for heterogeneity detection in TMI.

Heterogeneity Detection Method for Transmission Multispectral Imaging Based on Contour and Spectral Features