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Single-drop microextraction (SDME) was coupled with surface-enhanced Raman scattering (SERS) to provide sample extraction and pre-concentration for detection of analyte at low concentrations. A gold nanohole array substrate (AuNHAS), fabricated by interference lithography, was used as SERS substrate and para-mercaptobenzoic acid (p-MBA) was tested as a probe molecule, in the concentration range 10 -8 -10 -4 mol L -1 . With this approach, a limit of 10 -7 mol L -1 was clearly detected. To improve the detection to lower p-MBA concentration, as 10 -8 mol L -1 , the SDME technique was applied. The p-MBA Raman signature was detected in two performed extractions and its new concentration was determined to be ~4.6 × 10 -5 mol L -1 . This work showed that coupling SDME with SERS allowed a rapid (5 min) and efficient pre-concentration (from 10 -8 mol L -1 to 10 -5 mol L -1 ), detection, and quantification of the analyte of interest, proving to be an interesting analytical tool for SERS applications.

Coupling Single-Drop Microextraction with SERS: A Demonstration Using p-MBA on Gold Nanohole Array Substrate