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Geometric dimensions of plants are significant parameters for showing plant dynamic responses to environmental variations. An image-based high-throughput phenotyping platform was developed to automatically measure geometric dimensions of plants in a greenhouse. The goal of this paper was to evaluate the accuracy in geometric measurement using the Structure from Motion (SfM) method from images acquired using the automated image-based platform. Images of nine artificial objects of different shapes were taken under 17 combinations of three different overlaps in x and y directions, respectively, and two different spatial resolutions ( SR s) with three replicates. Dimensions in x , y and z of these objects were measured from 3D models reconstructed using the SfM method to evaluate the geometric accuracy. A metric power of unit ( POU) was proposed to combine the effects of image overlap and SR . Results showed that measurement error of dimension in z is the least affected by overlap and SR among the three dimensions and measurement error of dimensions in x and y increased following a power function with the decrease of POU ( R ² = 0.78 and 0.88 for x and y respectively). POU s from 150 to 300 are a preferred range to obtain reasonable accuracy and efficiency for the developed image-based high-throughput phenotyping system. As a study case, the developed system was used to measure the height of 44 plants using an optimal POU in greenhouse environment. The results showed a good agreement ( R ² = 92% and Root Mean Square Error = 9.4 mm) between the manual and automated method.

Evaluating Geometric Measurement Accuracy Based on 3D Reconstruction of Automated Imagery in a Greenhouse