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The reagent Rh-C, incorporating a rhodamine moiety and a coumarin backbone and prepared via click chemistry, was developed as the first single molecule for detecting Cu2+, Fe3+ and Cr3+. Its response to Cu2+ in different solutions is visible to the naked eye and it exhibits a ratiometric fluorescence response to Fe3+ in methanol and Cr3+ in acetonitrile.

One Single Molecule as a Multifunctional Fluorescent Probe for Ratiometric Sensing of Fe3+, Cr3+ and Colorimetric Sensing of Cu2+