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A meticulous image processing workflow is oftentimes required to derive quality image data from high-resolution, unmanned aerial systems. There are many subjective decisions to be made during image processing, but the effects of those decisions on prediction model accuracy have never been reported. This study introduced a framework for quantifying the effects of image processing methods on model accuracy. A demonstration of this framework was performed using high-resolution hyperspectral imagery (&lt;10 cm pixel size) for predicting maize nitrogen uptake in the early to mid-vegetative developmental stages (V6–V14). Two supervised regression learning estimators (Lasso and partial least squares) were trained to make predictions from hyperspectral imagery. Data for this use case were collected from three experiments over two years (2018–2019) in southern Minnesota, USA (four site-years). The image processing steps that were evaluated include (i) reflectance conversion, (ii) cropping, (iii) spectral clipping, (iv) spectral smoothing, (v) binning, and (vi) segmentation. In total, 648 image processing workflow scenarios were evaluated, and results were analyzed to understand the influence of each image processing step on the cross-validated root mean squared error (RMSE) of the estimators. A sensitivity analysis revealed that the segmentation step was the most influential image processing step on the final estimator error. Across all workflow scenarios, the RMSE of predicted nitrogen uptake ranged from 14.3 to 19.8 kg ha−1 (relative RMSE ranged from 26.5% to 36.5%), a 38.5% increase in error from the lowest to the highest error workflow scenario. The framework introduced demonstrates the sensitivity and extent to which image processing affects prediction accuracy. It allows remote sensing analysts to improve model performance while providing data-driven justification to improve the reproducibility and objectivity of their work, similar to the benefits of hyperparameter tuning in machine learning applications.

The Influence of Aerial Hyperspectral Image Processing Workflow on Nitrogen Uptake Prediction Accuracy in Maize