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Nitrogen (N) splitting is critical to achieving high crop yields without having negative effects on the environment. Monitoring crop N status throughout the wheat growing season is key to finding the balance between crop N requirements and fertilizer needs. Three soft winter wheat fertilization trials under rainfed conditions in Mediterranean climate conditions were monitored with a RapidScan CS-45 (Holland Scientific, Lincoln, NE, USA) instrument to determine the normalized difference vegetation index (NDVI) values at the GS30, GS32, GS37, and GS65 growth stages. The threshold NDVI values in the Cezanne variety were 0.7–0.75 at the GS32, GS37, and GS65 growing stages. However, for the GS30 growing stage, a threshold value could not be established precisely. At this stage, N deficiency may not affect wheat yield, as long as the N status increases at GS32 stage and it is maintained thereafter. Following the NDVI dynamic throughout the growing season could help to predict the yields at harvest time. Therefore, the ΣNDVI from GS30 to GS65 explains about 80% of wheat yield variability. Therefore, a given yield could be achieved with different dynamics in wheat NDVI values throughout the growing cycle. The determined ranges of the NDVI values might be used for developing new fertilization strategies that are able to adjust N fertilization to wheat crop needs.

Wheat Yield Estimation with NDVI Values Using a Proximal Sensing Tool