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Earth observation data can provide important information for tree species diversity mapping and monitoring. The relatively recent advances in remote sensing data characteristics and processing systems elevate the potential of satellite imagery for providing accurate, timely, consistent, and robust spatially explicit estimates of tree species diversity over forest ecosystems. This study was conducted in Northern Pindos National Park, the largest terrestrial park in Greece and aimed to assess the potential of four satellite sensors with different instrumental characteristics, for the estimation of tree diversity. Through field measurements, we originally quantified two diversity indices, namely the Shannon diversity index (H’) and Simpson’s diversity (D1). Random forest regression models were developed for associating remotely sensed spectral signal with tree species diversity within the area. The models generated from the use of the WorldView-2 image were the most accurate with a coefficient of determination of up to 0.44 for H’ and 0.37 for D1. The Sentinel-2 -based models of tree species diversity performed slightly worse, but were better than the Landsat-8 and RapidEye models. The coefficient of variation quantifying internal variability of spectral values within each plot provided little or no usage for improving the modelling accuracy. Our results suggest that very-high-spatial-resolution imagery provides the most important information for the assessment of tree species diversity in heterogeneous Mediterranean ecosystems.

A Random Forest Modelling Procedure for a Multi-Sensor Assessment of Tree Species Diversity