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Accurate digital mapping of soil organic carbon (SOC) is important in understanding the global carbon cycle and its implications in mitigating climate change. Visible and near-infrared hyperspectral imaging technology provides an alternative for mapping SOC efficiently and accurately, especially at regional and global scales. However, there is a lack of understanding of the impacts of spatial resolution of hyperspectral images and spatial autocorrelation of spectral information on the accuracy of SOC retrievals. In this study, the hyperspectral images (380–1700 nm) with a spatial resolution of 1 m were acquired by Headwall Micro-Hyperspec airborne sensors. Then, hyperspectral images were resampled into three different spatial resolutions of 10 m, 30 m, and 60 m by near neighbor (NN), bilinear interpolation (BI), and cubic convolution (CC) resampling methods. The geographically weighted regression (GWR) model was used to explore the role of spatial autocorrelation in predicting SOC contrast with the partial least squares regression (PLSR) model. Results showed that (1) the hyperspectral images can be used to predict SOC and the spatial autocorrelation can improve the prediction accuracy, as the ratio of performance to interquartile range (RPIQ) values of PLSR and GWR were 1.957 and 2.003; (2) The SOC prediction accuracy decreased with the degradation of spatial resolution, and the RPIQ values of PLSR were from 1.957 to 1.134, and of GWR were from 2.003 to 1.136; (3) Three resampling methods had a much weaker influence than spatial resolution on SOC predictions because the differences of RPIQ values of NN, BI, and CC resampling methods were 0.146, 0.175, and 0.025 in the spatial resolutions of 10 m, 30 m, and 60 m, respectively; (4) Finally, the Global Moran’s I and the Anselin Local Moran’s I proved the existence of the spatial autocorrelation in SOC maps. We hope that this study can offer valuable information for digital soil mapping by satellite hyperspectral images in the near future.

Exploring the Influence of Spatial Resolution on the Digital Mapping of Soil Organic Carbon by Airborne Hyperspectral VNIR Imaging