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Nanoplastics (NPs) are a rapidly developing subject that is relevant in environmental and food research, as well as in human toxicity, among other fields. NPs have recently been recognized as one of the least studied types of marine litter, but potentially one of the most hazardous. Several studies are now being reported on NPs in the environment including surface water and coast, snow, soil and in personal care products. However, the extent of contamination remains largely unknown due to fundamental challenges associated with isolation and analysis, and therefore, a methodological gap exists. This article summarizes the progress in environmental NPs analysis and makes a critical assessment of whether methods from nanoparticles analysis could be adopted to bridge the methodological gap. This review discussed the sample preparation and preconcentration protocol for NPs analysis and also examines the most appropriate approaches available at the moment, ranging from physical to chemical. This study also discusses the difficulties associated with improving existing methods and developing new ones. Although microscopical techniques are one of the most often used ways for imaging and thus quantification, they have the drawback of producing partial findings as they can be easily mixed up as biomolecules. At the moment, the combination of chemical analysis (i.e., spectroscopy) and newly developed alternative methods overcomes this limitation. In general, multiple analytical methods used in combination are likely to be needed to correctly detect and fully quantify NPs in environmental samples.

New Analytical Approaches for Effective Quantification and Identification of Nanoplastics in Environmental Samples