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Microbial fuel cells (MFCs) are a recent biotechnology that can simultaneously produce electricity and treat wastewater. As the nature of industrial wastewater is very complex, and it may contain a variety of substrates—such as carbohydrates, proteins, lipids, etc.—previous investigations dealt with treatment of individual pollutants in MFCs; the potential of acetic acid, sucrose, albumin, blood, and their mixture has rarely been reported. Hence, the current investigation explored the contribution of each substrate, both separately and in mixture. The voltage generation potential, current, and power density of five different substrates—namely, acetic acid, sucrose, albumin, blood, and a mixture of all of the substrates—was tested in a dual-chambered, anaerobic MFC operated at 35 °C. The reaction time of the anaerobic batch mode MFC was 24 h, and each substrate was treated for 7 runs under the same conditions. The dual-chambered MFC consisted of anode and cathode chambers; the anode chamber contained the biocatalyst (sludge), while the cathode chamber contained the oxidizing material (KMnO4). The maximum voltage of 769 mV was generated by acetic acid, while its corresponding values of current and power density were 7.69 mA and 347.85 mW, respectively. Similarly, being a simple and readily oxidizable substrate, acetic acid exhibited the highest COD removal efficiency (85%) and highest Coulombic efficiency (72%) per run. The anode accepted the highest number of electrons (0.078 mmol/L) when acetic acid was used as a substrate. The voltage, current, and power density generated were found to be directly proportional to COD concentration. The least voltage (61 mV), current (0.61 mA), and power density (2.18 mW) were observed when blood was treated in the MFC. Further research should be focused on testing the interaction of two or more substrates simultaneously in the MFC.

Bioenergy Potential of Albumin, Acetic Acid, Sucrose, and Blood in Microbial Fuel Cells Treating Synthetic Wastewater