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This paper presents the complete development and experimental validation of a microsystem designed to systematically assess the impact of surface topography on cell adhesion and dynamics. The microsystem includes two pools for culturing cells and for including chemicals. These pools are connected by several channels that have different microtextures, along which the cells crawl from one well to another. The impact of channel surface topography on cell performance, as well as the influence of other relevant factors, can therefore be assessed. The microsystem stands out for its being able to precisely define the surface topographies from the design stage and also has the advantage of including the different textures under study in a single device. Validation has been carried out by culturing human mesenchymal stem cells (hMSCs) on the microsystem pre-treated with a coating of hMSC conditioned medium (CM) produced by these cells. The impact of surface topography on cell adhesion, motility, and velocity has been quantified, and the relevance of using a coating of hMSC-CM for these kinds of studies has been analyzed. Main results, current challenges, and future proposals based on the use of the proposed microsystem as an experimental resource for studying cell mechanobiology are also presented.This work was carried out with the support of the European Community. We appreciate the support of the European Research Infrastructure EUMINAfab (funded under the FP7 specific programme Capacities, Grant Agreement Number 226460) and its partner the Karlsruhe Institute of Technology. We are also grateful to Dieter Maas and to Thomas Schaller for their kind help and support for the EUMINAfab 1099 proposal on “Replication of advanced scaffolds with biomimetic fractal features”. This work was partly carried out with the support of the Karlsruhe Nano Micro Facility (KNMF, www.knmf.kit.edu), the Helmholtz Research Infrastructure at Karlsruhe Institute of Technolog

Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility