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The effects of hydrothermal pretreatment (170⁻180 °C, 30⁻60 min) on the structural characteristics of enzymatic and extracted lignin from Triarrhena lutarioriparia ( TL ) during the integrated delignification process have been comprehensively investigated. Ion chromatography and NMR characterization showed that liquid products after mild hydrothermal process (170 °C, 30 min) were mainly composed of xylooligosaccharide (XOS) with different degrees of polymerization (DP ≥ 2). In addition, the structural changes of lignin during hydrothermal pretreatment and organic acid delignification process have been demonstrated by quantitative 2D heteronuclear single quantum coherence (2D-HSQC) and 31 P-NMR techniques. Results showed that the structural changes of lignin (e.g., cleavage of β- O -4 linkages) induced by the hydrothermal pretreatment will facilitate the subsequent organic acid delignification process, and acetylated lignin could be obtained with a considerable yield, which can be used in lignin-based composite and candidate feedstock for catalytic upgrading of lignin. In short, the proposed process facilitates the producing of XOS and acetylated lignin for lignin valorization.

Effects of Hydrothermal Pretreatment on the Structural Characteristics of Organosolv Lignin from Triarrhena lutarioriparia