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In this paper, chitosan (CS)/graphene oxide (GO) composite films were prepared, and the effect of γ-ray irradiation on the properties of composite films was investigated. The irradiation crosslinking reaction occurred in composite films with the existence of acetic acid, and the properties changed upon the various irradiation dose. The swelling degree of the composite film with 0 wt % GO decreased with the increasing of the irradiation dose, but the swelling degree of which with GO increased instead. The thermal stability increased with the increasing of the irradiation dose, but the effect of the irradiation on the thermal stability weakened as the increasing of the content of GO, due to the enhanced irradiation resistance performance. The tensile strength increased firstly and decreased subsequently with the increasing of the irradiation dose and the content of GO. Composite films showed the enhanced antibacterial activity against Bacillus subtilis , compared to Escherichia coli and Staphylococcus aureus . The antibacterial activity weakened with the increasing of the content of GO. The antibacterial activity was relatively stronger when the irradiation dose was 20 KGy. In addition, the structural, crystal, and morphological properties of composite films were characterized by FT-IR, XRD, and SEM. It is worth noting that the GO was pre-functionalized via KH560 for the better compatibility with CS matrix.

60Co γ-ray Irradiation Crosslinking of Chitosan/Graphene Oxide Composite Film: Swelling, Thermal Stability, Mechanical, and Antibacterial Properties