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Efficient nitrogen (N) nutrition has been reported to have the potential to alleviate the drought stress damages by maintaining metabolic activities even at low tissue water potential. The goal of our research was to find a correlation on the genotype level between the effect of different amounts of nitrogen nutrition and water supply at different growth stages. A small-plot experiment was established with three maize hybrids and three levels of nitrogen, and two different amounts of water supply were applied during the vegetation period of 2018 and 2019. Chlorophyll fluorescence parameters were detected, as well as potential and actual photochemical efficiency of PSII, at three growth stages: eight-leaf stage, tasseling, silking. At physiological maturity, the yield of hybrids was also measured. While only genotype differences were described among the investigated parameters in the V8 stage, treatment effects were also realized based on the measured chlorophyll fluorescence parameters during the tasseling and silking stages. Beyond the significant effect of irrigation, a similar impact was declared in the case of 80 kg ha -1 N treatment at the later growth stages. Pronounced correlation was described between chlorophyll fluorescence parameters and yield mainly under irrigated conditions. Our result suggested that lower N nutrition may be sufficient mainly under irrigated conditions, and in vivo chlorophyll fluorescence parameters are appropriate for detecting the effect of environmental factors in different growth stages.

Evaluation of Nitrogen Nutrition in Diminishing Water Deficiency at Different Growth Stages of Maize by Chlorophyll Fluorescence Parameters