English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Calnexin (CNX) is an integral membrane protein that functions as a chaperone in the endoplasmic reticulum for the correct folding of proteins under stress conditions, rendering organisms tolerant under adverse conditions. Studies have investigated the cytogenetic effects of gamma irradiation (Ɣ-IR) on plants, but information on the molecular response under Ɣ-IR remains limited. Previously, we constructed a cDNA library of an irradiation-sensitive bioindicator plant,  Tradescantia  BNL4430 (T-4430) under Ɣ-IR, in which the  Calnexin-1  gene was highly upregulated at 50 mGy treatment.  TrCNX1  encodes a 61.4 kDa protein with conserved signature motifs similar to already reported  CNX1 s.  TrCNX1  expression was evaluated by semiquantitative reverse transcriptase PCR and quantitative real-time PCR and was ubiquitously expressed in various tissues and highly upregulated in flower petals under 50 mGy Ɣ-IR stress. The protective function of  TrCNX1  was investigated by overexpression of TrCNX1 in an  Escherichia coli  BL21(DE3) heterologous system. Using plate assay, we showed that TrCNX1 increased the viability of  E .  coli  transformants under both UV-B and Ɣ-IR compared with the control, demonstrating that  TrCNX1  functions under irradiation stress.  TrCNX1  may enhance irradiation stress tolerance in crops and act as a radio marker gene to monitor the effects of radiation.

Molecular Characterization and Identification of Calnexin 1 As a Radiation Biomarker from Tradescantia BNL4430