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Determination of S status is very important to detect S deficiency and prevent losses of yield and seed quality. The aim of this study was to investigate the possibility of using the ([Cl &amp;minus; ]+[NO₃ &amp;minus; ]+[PO₄ 3&amp;minus; ]):[SO₄ 2&amp;minus; ] ratio as an indicator of S nutrition under field conditions in Brassica napus and whether this could be applied to other species. Different S and nitrogen (N) fertilizations were applied on a S deficient field of oilseed rape to harvest mature leaves and analyze their anion and element contents in order to evaluate a new S nutrition indicator and useful threshold values. Large sets of commercial varieties were then used to test S deficiency scenarios. As main results, this study shown that, under field conditions, leaf ([Cl &amp;minus; ]+[NO₃ &amp;minus; ]+[PO₄ 3&amp;minus; ]):[SO₄ 2&amp;minus; ] ratio was increased by lowering S fertilization, indicating S deficiency. The usefulness of this ratio was also found for other species grown under controlled conditions and it could be simplified by using the elemental ([Cl]+[P]):[S] ratio. Threshold values were determined and used for the clustering of commercial varieties within three groups: S deficient, at risk of S deficiency and S sufficient. The ([Cl]+[P]):[S] ratio quantified under field conditions, can be used as an early and accurate diagnostic tool to manage S fertilization.

Assessment of Sulfur Deficiency under Field Conditions by Single Measurements of Sulfur, Chloride and Phosphorus in Mature Leaves