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The in vitro antioxidant potential assay between ethanolic extracts of two species from the genus  Vitex  ( Vitex negundo  L. and  Vitex trifolia  L.) belonging to the Lamiaceae family were evaluated. The antioxidant properties of different extracts prepared from both plant species were evaluated by different methods. DPPH scavenging, nitric oxide scavenging, and β-carotene-linoleic acid and ferrous ion chelation methods were applied. The antioxidant activities of these two species were compared to standard antioxidants such as butylated hydroxytoluene (BHT), ascorbic acid, and Ethylene diamine tetra acetic acid (EDTA). Both species of  Vitex  showed significant antioxidant activity in all of the tested methods. As compared to  V. trifolia  L. (60.87–89.99%; 40.0–226.7 μg/mL),  V. negundo  has been found to hold higher antioxidant activity (62.6–94.22%; IC 50  = 23.5–208.3 μg/mL) in all assays. In accordance with antioxidant activity, total polyphenol contents in  V. negundo  possessed greater phenolic (89.71 mg GAE/g dry weight of extract) and flavonoid content (63.11 mg QE/g dry weight of extract) as compared to that of  V. trifolia  (77.20 mg GAE/g and 57.41 mg QE/g dry weight of extract respectively). Our study revealed the significant correlation between the antioxidant activity and total phenolic and flavonoid contents of both plant species.

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Comparative Evaluation of Polyphenol Contents and Antioxidant Activities between Ethanol Extracts of Vitex negundo and Vitex trifolia L. Leaves by Different Methods