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To elucidate the chemical compositions of the aerial parts of Chrysanthemum coronarium L., the ethanol extracts of Ch. coronarium L. were firstly isolated by the MCI-gel resin column. The caffeoylquinic acid-rich fractions were further purified by various chromatographic columns including silica gel, Sephadex LH-20, and semi-preparative HPLC to yield the compounds. The purified compounds were characterized by ¹H-Nuclear Magnetic Resonance (¹H-NMR), 13 C-NMR, and high resolution electrospray ionisation mass spectral (HR-ESI-MS) spectroscopy. Seven caffeoylquinic acid (CQA) compounds were isolated from this plant. Their structures were clarified by spectrometric methods and identified as 3- O -caffeoylquinic acid ( 1 ), 5- O -caffeoylquinic acid ( 2 ), 4- O -caffeoylquinic acid ( 3 ), 3,4-di- O -caffeoylquinic acid ( 4 ), 1,5-di- O -caffeoylquinic acid ( 5 ), 3,5-di- O -caffeoylquinic acid ( 6 ), and 4,5-di- O -caffeoylquinic acid ( 7 ). Caffeoylquinic acids were the major constituents present in the aerial parts of Ch. coronarium L. All of the isolates except for compounds 2 and 6 were reported for the first time from this species. Moreover, compounds 3 - 5 , and 7 were identified from the Chrysanthemum genus for the first time.

Caffeoylquinic Acids from the Aerial Parts of Chrysanthemum coronarium L.