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Rhus michauxii  is a perennial rhizomatous shrub native to the southeastern United States that is found mainly in sunny, dry, open rocky or sandy woodlands. Moreover, it is found on ridges or river bluffs in the inner coastal plane and lower piedmont of Virginia, Georgia, and the Carolinas. Habitat conversion to agriculture, suppression of fires, and low reproduction have caused  R. michauxii  to become rare and it is now federally listed as threatened. Methods are needed to multiply and conserve  R. michauxii . Protocols were developed for seed cryopreservation, in vitro germination, and micropropagation for  R. glabra  and  R. michauxii . Seed scarification in concentrated sulfuric acid for 6 h and germination on ½ MS medium resulted in germination up to 96% for control and cryopreserved seeds of  R. glabra  and 70 and 40% for control and cryopreserved seeds of  R. michauxii . Shortly after germination in vitro, young seedlings were established in a greenhouse potting mix providing new plants from the endemic Georgia  R. michauxii  populations. Several of the findings meet goals within the  R. michauxii  recovery plan by providing methods for sexual and asexual multiplication and long-term seed storage under cryogenic conditions. The protocols developed will assist in the safeguarding and conservation of dwindling natural  R. michauxii  populations.

Seed Cryopreservation and Germination of Rhus glabra and the Critically Endangered Species Rhus michauxii