English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We presented an approach to address cancer cell chemotaxis and response to tyrosine kinase inhibitor PD153035 at the single-cell level. We applied an optical tweezer system together with the platform at the single-cell level to manipulate an epidermal growth factor (EGF)-coated bead positioned close to the filopodia to locally stimulate HT29 cells, the human colon cancer cell line overexpressing the EGF receptor (EGFR). To address cancer cell chemotaxis, a single-cell movement model was also proposed to quantify the propagation speed at the leading and trailing edges of the cell along the chemosensing axis. This study focused on three perspectives: probing the chemosensing process mediated by EGF/EGFR signaling, investigating the mode of locomotion during the EGF-coated bead stimulation, and quantifying the effect of PD153035 on the EGF–EGFR transport pathway. The results showed that the filopodial actin filament is a sensory system for EGF detection. In addition, HT29 cells may use the filopodial actin filament to distinguish the presence or absence of the chemoattractant EGF. Furthermore, we demonstrated the high selectivity of PD153035 for EGFR and the reversibility of binding to EGFR. We anticipate that the proposed single-cell method could be applied to construct a rapid screening method for the detection and therapeutic evaluation of many types of cancer during chemotaxis.

photonics

An Optical Tweezers-Based Single-Cell Manipulation and Detection Platform for Probing Real-Time Cancer Cell Chemotaxis and Response to Tyrosine Kinase Inhibitor PD153035