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The most realistic information about the transparent sample such as a livecell can be obtained only using bright-field light microscopy. Athigh-intensity pulsing LED illumination, we captured a primary12-bit-per-channel (bpc) response from an observed sample using a bright-fieldmicroscope equipped with a high-resolution (4872x3248) image sensor. In orderto suppress data distortions originating from the light interactions withelements in the optical path, poor sensor reproduction (geometrical defects ofthe camera sensor and some peculiarities of sensor sensitivity), we propose aspectroscopic approach for the correction of this uncompressed 12-bpc data bysimultaneous calibration of all parts of the experimental arrangement.Moreover, the final intensities of the corrected images are proportional to thephoton fluxes detected by a camera sensor. It can be visualized in 8-bpcintensity depth after the Least Information Loss compression.

Spectroscopic Approach to Correction and Visualisation of Bright-Field Light Transmission Microscopy Biological Data