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The as-prepared co-doped N/Ag carbon dot (CD NAg ) has strong catalysis of H₂O₂ oxidation of 3,3'-dimethylbiphenyl-4,4'-diamine (DBD). It forms an oxidation product (DBD ox ) with surface-enhanced Raman scattering (SERS) activity at 1605 cm -1 in the silver nanosol substrate, and a CD NAg catalytic amplification with SERS analytical platform can be structured based on aptamer (Apt) with the DBD oxidizing reaction. For example, the aptamer (Apt) of acetamiprid (ACT) can be adsorbed on the surface of CD NAg , resulting in inhibited catalytic activity, the reduced generation of DBD ox , and a weakened SERS intensity. When the target molecule ACT was added, it formed a stable Apt-ACT complex and free CD NAg that restored catalytic activity and linearly enhanced the SERS signal. Based on this, we proposed a new quantitative SERS analysis method for the determination of 0.01⁻1.5 μg ACT with a detection limit of 0.006 μg/L.

Doped N/Ag Carbon Dot Catalytic Amplification SERS Strategy for Acetamiprid Coupled Aptamer with 3,3′-Dimethylbiphenyl-4,4′-diamine Oxidizing Reaction