English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Prion protein (PrP Sc ) has drawn widespread attention due to its pathological potential to prion diseases. In this work, we constructed a novel surface plasmon resonance (SPR) detection assay involving magnetic microspheres (MMs) and its controlled release property, for selective capture, embedding, concentration, and SPR detection of PrP Sc with high sensitivity and specificity. Aptamer-modified magnetic particles (AMNPs) were used to specifically capture PrP Sc . Amphiphilic copolymer was used to embed the labeled PrP Sc and form magnetic microspheres to isolate PrP Sc from the external environment. Static magnetic and alternating magnetic fields were used to concentrate and control release the embedded PrP Sc , respectively. Finally, the released AMNPs-labeled PrP Sc was detected by SPR which was equipped with a bare gold sensing film. A good linear relationship was obtained between SPR responses and the logarithm of PrP Sc concentrations over a range of 0.01-1000 ng/mL. The detection sensitivity for PrP Sc was improved by 10 fold compared with SPR direct detection format. The specificity of the present biosensor was also determined by PrP C and other reagents as controls. This proposed approach could also be used to isolate and detect other highly pathogenic biomolecules with similar structural characteristics by altering the corresponding aptamer in the AMNPs conjugates.

nanomaterials

Qualitative and Quantitative Detection of PrPSc Based on the Controlled Release Property of Magnetic Microspheres Using Surface Plasmon Resonance (SPR)