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The enzyme mimetic activity of nanomaterials has been applied in colorimetric assays and point-of-care diagnostics. Several nanomaterials have been exploited for their peroxidase mimetic activity toward 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. However, an efficient nanomaterial for the rapid and strong oxidation of TMB remains a strategic challenge. Therefore, in this study, we developed copper-loaded tin oxide (SnO 2 -Cu) nanocomposites that rapidly oxidize TMB. These nanocomposites have strong absorption at 650 nm and can be used for highly sensitive colorimetric detection. An environmentally friendly (green), rapid, easy, and cost-effective method was developed for the synthesis of these nanocomposites, which were characterized using ultraviolet-visible, energy-dispersive X-ray, and Fourier-transform infrared spectroscopy, as well as scanning electron microscopy. This is the first green synthesis of SnO 2 -Cu nanocomposites. Their enzyme mimetic activity, which was first studied here, was found to be strongly dependent on the temperature and pH value of the solution. The synthesized nanocomposites have the advantages of low cost, high stability, and ease of preparation for enzyme mimetic applications. Hence, SnO 2 -Cu nanocomposites are a promising alternative to peroxidase enzymes in colorimetric point-of-care diagnostics.

Eco-Friendly Synthesis of SnO2-Cu Nanocomposites and Evaluation of Their Peroxidase Mimetic Activity