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The present study investigated the biosynthesis of silver (AgNPs), zinc oxide (ZnONPs) and titanium dioxide (TiO 2 NPs) nanoparticles using  Aspergillusoryzae ,  Aspergillusterreus  and  Fusariumoxysporum . Nanocomposites (NCs) were successfully synthesized by mixing nanoparticles using a Sonic Vibra-Cell VC/VCX processor. A number of analytical techniques were used to characterize the synthesized biological metal nanoparticles. Several experiments tested biologically synthesized metal nanoparticles and nanocomposites against two types of human pathogenic bacteria, including Gram-positive  Staphylococcus aureus  and methicillin-resistant  Staphylococcus aureus  (MRSA), and Gram-negative  Escherichia coli  and  Pseudomonasaeruginosa . Additionally, the antitumor activity in HCT-116 cells (colonic carcinoma) was also evaluated. Significant antimicrobial effects of various synthesized forms of nanoparticles and nanocomposites against  E. coli  and  P. aeruginosa  bacteria were detected. Various synthesized biogenic forms of nanoparticles and nanocomposite (9.0 to 29 mm in diameter) had high antibacterial activity and high antitumor activity against HCT-116 cells (colonic carcinoma) with IC 50  values of 0.7–100 µg/mL. Biosynthesized NPs are considered an alternative to large-scale biosynthesized metallic nanoparticles and nanocomposites, are simple and cost effective, and provide stable nanomaterials.

Interactive Effects of Biosynthesized Nanocomposites and Their Antimicrobial and Cytotoxic Potentials