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In this work CeO 2 nanoparticles (CeO 2 -NPs) were synthesized through the thermal decomposition of Ce(NO 3 ) 3 ·6H 2 O, using as capping agents either octylamine or oleylamine, to evaluate the effect of alkyl chain length, an issue at 150 °C, in the case of octylamine and at 150 and 250 °C, in the case of oleylamine, to evaluate the effect of the temperature on NPs properties. All the nanoparticles were extensively characterized by a multidisciplinary approach, such as wide-angle X-ray diffraction, transmission electron microscopy, dynamic light scattering, UV-Vis, fluorescence, Raman and FTIR spectroscopies. The analysis of the experimental data shows that the capping agent nature and the synthesis temperature affect nanoparticle properties including size, morphology, aggregation and Ce 3+ /Ce 4+ ratio. Such issues have not been discussed yet, at the best of our knowledge, in the literature. Notably, CeO 2 -NPs synthesized in the presence of oleylamine at 250 °C showed no tendency to aggregation and we made them water-soluble through a further coating with sodium oleate. The obtained nanoparticles show a less tendency to clustering forming stable aggregates (ranging between 14 and 22 nm) of few NPs. These were tested for biocompatibility and ROS inhibiting activity, demonstrating a remarkable antioxidant activity, against oxidative stress.

nanomaterials

Towards the Development of Antioxidant Cerium Oxide Nanoparticles for Biomedical Applications: Controlling the Properties by Tuning Synthesis Conditions