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In nearly all the cases of biotoxicity studies of silver nanoparticles (AgNPs), AgNPs used often have general dispersibility and wide size distribution, which may inevitably generate imprecise results. Herein, a kind of synthesis method by coupling effects of polyvinylpyrrolidone (PVP) and sodium tripolyphosphate (STPP) was proposed, in order to prepare AgNPs with better dispersibility and a stable size. Based on this, the preparation mechanism of AgNPs and the potential anti-algae toxicity were analyzed. UV-vis analysis showed that the particle size distribution of AgNPs prepared by co-protective agents was more uniform. X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), and energy dispersive X-ray (EDX) were used to confirm that the obtained nano silver was of a high purity and stable size (~30 nm in diameter). Zeta potential and Fourier transform infrared spectroscopy (FTIR) analysis results indicated the synthesis mechanism of AgNPs by co-protective agents, more precisely, PVP limited the polynegative effect and prevented the linear induction of P 3 O 10  5  - produced by STPP during the growth of silver nuclei. Subsequently, Chlorella and Scenedesmus obliquus were utilized to test the toxicity of AgNPs, confirming that AgNPs synthesized through co-protective agents have potential inhibitory ability on algae, but not severe. This study provides a basic theory for the induction of synthetic AgNPs by various factors in the natural environment and a scientific reference for the environmental risk assessment.

nanomaterials

Dispersibility and Size Control of Silver Nanoparticles with Anti-Algal Potential Based on Coupling Effects of Polyvinylpyrrolidone and Sodium Tripolyphosphate