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This work presents, for the first time, a new sequential injection analysis (SIA) method to simultaneously analyze degree Brix, orthophosphate and pH in raw cane juice. These key parameters relate to price of harvested sugarcane and quality of cane juice for sugar production. The SIA system employed two detectors: the first detector is a diode-array spectrophotometer, equipped with a regular flow cell, for measurements of degree Brix and orthophosphate. Quantitative of degree Brix (°Bx; ca. % ( w / w ) sucrose) was based on manipulation of the schlieren effect at the interface between plugs of sample and water. Orthophosphate analysis was carried out based on the molybdenum blue method with significant reduction in consumption of the reagents. Compensation of the schlieren effect from sucrose for determination of orthophosphate was achieved by using a dual-wavelength spectrometric detection. Second detector is a pH-sensing device, called ion-selective field-effect transistors (ISFET). The ISFET is based on the current through the ISFET arising according to the H +  concentration in solution. Our developed SIA system provides linear calibration graphs fitting for purpose in analysis of sugarcane juice (pH: 0–14, °Bx: 1.0–7.0 and P 2 O 5 : 20–200 mg L −1 ). Simultaneous analysis of sugarcane juice for pH, °Bx and P 2 O 5  is carried out within 5 min (12 sample per h). Precision of SIA system is acceptable (RSD < 3%). Our SIA system gave quantitative results insignificantly different, as compared with conventional methods for analysis of pH, °Bx and P 2 O 5  in sugarcane juice.

Sequential Injection System for Analysis of Degree Brix, Orthophosphate and pH in Raw Sugarcane Juice Applicable to Sugar Industry