Open AccessAntimicrobial Peptide Dendrimers and Quorum-Sensing Inhibitors in Formulating Next-Generation Anti-Infection Cell Therapy Dressings for Burns
Author(s) -
Paris Jafari,
Alexandre Lüscher,
Thissa N. Siriwardena,
Murielle Michetti,
Yok-Ai Que,
Laurence G. Rahme,
JeanLouis Reymond,
Wassim Raffoul,
Christian van Delden,
Lee Ann Applegate,
Thilo Köhler
Publication year - 2021
Publication title -
molecules/molecules online/molecules annual
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 149
eISSN - 1433-1373
pISSN - 1420-3049
DOI - 10.3390/molecules26133839
Subject(s) - quorum sensing , antimicrobial , microbiology and biotechnology , virulence , progenitor cell , antimicrobial peptides , antibiotics , lysis , bacteria , dendrimer , antibiotic resistance , cell , viability assay , peptide , chemistry , biology , stem cell , immunology , biochemistry , genetics , gene
Multidrug resistance infections are the main cause of failure in the pro-regenerative cell-mediated therapy of burn wounds. The collagen-based matrices for delivery of cells could be potential substrates to support bacterial growth and subsequent lysis of the collagen leading to a cell therapy loss. In this article, we report the development of a new generation of cell therapy formulations with the capacity to resist infections through the bactericidal effect of antimicrobial peptide dendrimers and the anti-virulence effect of anti-quorum sensing MvfR (PqsR) system compounds, which are incorporated into their formulation. Anti-quorum sensing compounds limit the pathogenicity and antibiotic tolerance of pathogenic bacteria involved in the burn wound infections, by inhibiting their virulence pathways. For the first time, we report a biological cell therapy dressing incorporating live progenitor cells, antimicrobial peptide dendrimers, and anti-MvfR compounds, which exhibit bactericidal and anti-virulence properties without compromising the viability of the progenitor cells.