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This study evaluated the effect of seasonal variation on the physicochemical, biochemical, and nutritional composition of Gracilaria manilaensis . Sampling was designed during the main monsoon seasons in Malaysia-the Southwest monsoon (SWM) and Northeast monsoon (NEM)-to understand the intraspecific variation ( p &lt; 0.05). Carbohydrates, protein, and dietary fiber were found to be higher in NEM- G. manilaensis , whereas a higher ash content was quantified in SWM- G. manilaensis . No significant differences were found in crude lipid and moisture content ( p &gt; 0.05). Vitamin B2 was calculated as (0.29 ± 0.06 mg 100 g -1 ) and (0.38 ± 0.06 mg 100 g -1 ) for the NEM and SWM samples, respectively ( p &lt; 0.05). The fatty acid profile showed the dominance of saturated fatty acids (SFAs)-palmitic acids, stearic acid, and myristic acid-while the mineral contents were found to be good sources of calcium (1750.97-4047.74 mg 100 g -1 ) and iron (1512.55-1346.05 mg 100 g -1 ). Tryptophan and lysine were recorded as the limiting essential amino acids (EAAs) in NEM G. manilaensis , while leucine and phenylalanine were found to be the limiting EAAs in the SWM samples. None of the extracts exhibited antibacterial properties against the screened strains. The study concluded that seasonal changes have a great effect on the biochemical composition of G. manilaensis .

Effects of Seasonal Variability on the Physicochemical, Biochemical, and Nutritional Composition of Western Peninsular Malaysia Gracilaria manilaensis