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A new highly selective and sensitive fluorescent probe for Cu 2+ , N - n -butyl-4-(1'-cyclooctene-1',3',6'-triazole)-1,8-naphthalimide ( L ), was synthesized and evaluated. The structure of compound L was characterized via IR, ¹H-NMR, 13 C-NMR and HRMS. The fluorescent probe was quenched by Cu 2+ with a 1:1 binding ratio and behaved as a "turn-off" sensor. An efficient and sensitive spectrofluorometric method was developed for detecting and estimating trace levels of Cu 2+ in EtOH/H₂O. The ligand exhibited excitation and emission maxima at 447 and 518 nm, respectively. The equilibrium binding constant of the ligand with Cu 2+ was 1.57 × 10⁴ M -1 , as calculated using the Stern.

A Highly Selective and Sensitive Fluorescent Turn-Off Probe for Cu2+ Based on a Guanidine Derivative