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Two new phenolic glycosides, named gnaphaffine A and B (compounds 1 and 2), were isolated from Gnaphalium affine. together with six known compounds, including caffeic acid (3), everlastoside L (4), isorhamnetin-7-O-β-D-glucopyranoside (5), quercetin- 3-O-β-D-glucopyranoside (6), scutellarein-7-O-β-D-glucoside (7) and api-genin-7-O-β-D- glucopyranoside (8). Their structures were elucidated by spectroscopic methods, including ESI-MS and 2D NMR spectroscopy (HMQC and HMBC). All compounds were evaluated for their anti-complementary activity on the classical pathway of the complement system in vitro.

Two New Phenolic Glycosides from Gnaphalium affine D. Don and Their Anti-Complementary Activity