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Synthesis and characterization of Carbon nanotube/Hydroxyapatite/Clay based Hybrid antimicrobial biomaterial for potential tissue engineering application   Subrata Kara,b, Papiya Nandyb, Ruma Basub,c and Sukhen Das*a,b,d   aPhysics Department, Jadavpur University, Kolkata-700 032, India. bCentre for Interdisciplinary Research and Education, Kolkata 700 068, India cPhysics Department, Jogamaya Devi College, Kolkata 700 026 dIndian Institute of Engineering, Science and Technology, Howrah, India   *Corresponding author   Inorganic ceramic materials have recently been enjoying a great deal of attention for uses as biomaterial over the traditionally used polymeric material. The ability of silica based ceramics to release Si-containing ionic products makes them osteoconductive and their special surface composition has drawn considerable attention for its use as osteogenic proliferation, differentiation and gene expression of tissue cells. Here Pristine Multiwalled carbon nanotube (MWCNT) was functionalized by acid oxidation and used as template for nucleation of hydroxyapatite crystal. The CNT-HAP thus formed was further reinforced with exfoliated Montmoriollite(MMT) clay to increase the mechanical property of the nanocomposite. The synthesized composite viz CNT-HAP (HC) and CNT(HAP)-MMT(CHC) were characterized by fourier transform infrared spectroscopy(FTIR), X-ray diffraction(XRD), Scanning electron microscopy and transmission electron microscopy(TEM) and thermogravimetric analysis(TGA) . The biomedical application of CHC, blood compatibility was studied in terms of hemolysis assay and platelet adhesion assay. Finally, in vitro dissolution assay and the antibacterial activity of the material were further explored for establishing its use as an antibacterial biomaterial. The nanocomposite thus designed exhibited a promising candidate as an antimicrobial biomaterial for tissue Engineering application.

&lt;strong&gt;Synthesis and characterization of Carbon nanotube/Hydroxyapatite/Clay based Hybrid antimicrobial biomaterial for potential tissue engineering application&lt;/strong&gt;