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The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against  Mycobacterium bovis ,  Brucella suis , and  Trichinella spiralis . Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative),  Brucella  (30 seropositive, 39 seronegative), and  Trichinella  (21 seropositive, 97 seronegative) were used for the development and evaluation of the assay. Magnetic beads coated with recombinant MPB83 antigen (TB), a whole-cell  B. suis  1330 antigen, and an E/S  T. spiralis  antigen were used for the detection of specific antibodies using Bio-Rad Bio-Plex technology. The sensitivities (Se) and specificities (Sp) of the multiplex assay were, for  M. bovis , 0.98 and 0.86; for  B. suis , 1.00 and 0.97; and for  T. spiralis , 0.90 and 0.99 (Se and Sp, respectively). The results show the diagnostic potential of this assay for the simultaneous detection of antibodies against  M. bovis ,  B. suis , and  T. spiralis  in wild boar.

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Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar