English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The evolutionary epidemiology, resistome, virulome and mobilome of thirty-one multidrug resistant  Klebsiella pneumoniae  clinical isolates from the northern Vila Real region of Portugal were characterized using whole-genome sequencing and bioinformatic analysis. The genomic population structure was dominated by two main sequence types (STs): ST147 ( n  = 17; 54.8%) and ST15 ( n  = 6; 19.4%) comprising four distinct genomic clusters. Two main carbapenemase coding genes were detected ( bla  KPC-3  and  bla  OXA-48 ) along with additional extended-spectrum β-lactamase coding loci ( bla  CTX-M-15 ,  bla  SHV-12 ,  bla  SHV-27 , and  bla  SHV-187 ). Moreover, whole genome sequencing enabled the identification of one  Klebsiella variicola  KPC-3 producer isolate previously misidentified as  K. pneumoniae , which in addition to the  bla  KPC-3  carbapenemase gene, bore the chromosomal broad spectrum β-lactamase  bla  LEN-2  coding gene,  oqxAB  and  fosA  resistance loci. The  bla  KPC-3  genes were located in a  Tn4401b  transposon  (K. variicola   n  = 1;  K. pneumoniae   n  = 2) and  Tn4401d  isoform ( K. pneumoniae   n  = 28). Overall, our work describes the first report of a  bla  KPC-3  producing  K. variicola,  as well as the detection of this species during infection control measures in surveillance cultures from infected patients. It also highlights the importance of additional control measures to overcome the clonal dissemination of carbapenemase producing clones.

microorganisms

Genomic Epidemiology of Carbapenemase Producing Klebsiella pneumoniae Strains at a Northern Portuguese Hospital Enables the Detection of a Misidentified Klebsiella variicola KPC-3 Producing Strain