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Background : VIM (Verona Integron-encoded Metallo-beta-lactamase) is a member of the Metallo-Beta-Lactamases (MBLs), and is able to hydrolyze all beta-lactams antibiotics, except for monobactams, and including carbapenems. Here we characterize a VIM-producing IncA plasmid isolated from a clinical ST69 Escherichia coli strain from an Italian Long-Term Care Facility (LTCF) inpatient. Methods : An antimicrobial susceptibility test and conjugation assay were carried out, and the transferability of the bla  VIM-type gene was confirmed in the transconjugant. Whole-genome sequencing (WGS) of the strain 550 was performed using the Sequel I platform. Genome assembly was performed using "Microbial Assembly". Genomic analysis was conducted by uploading the contigs to ResFinder and PlasmidFinder databases. Results: Assembly resulted in three complete circular contigs: the chromosome (4,962,700 bp), an IncA plasmid (p550_IncA_VIM_1; 162,608 bp), harboring genes coding for aminoglycoside resistance ( aac(6')-Ib4 , ant(3″)-Ia , aph(3″)-Ib , aph(3')-XV , aph(6)-Id ), beta-lactam resistance ( bla  SHV-12 , bla  VIM-1 ), macrolides resistance ( mph(A) ), phenicol resistance ( catB2 ), quinolones resistance ( qnrS1 ), sulphonamide resistance ( sul1 , sul2 ), and trimethoprim resistance ( dfrA14 ), and an IncK/Z plasmid (p550_IncB_O_K_Z; 100,306 bp), free of antibiotic resistance genes. Conclusions: The increase in reports of IncA plasmids bearing different antimicrobial resistance genes highlights the overall important role of IncA plasmids in disseminating carbapenemase genes, with a preference for the bla  VIM-1 gene in Italy.

Genomic Insight of VIM-harboring IncA Plasmid from a Clinical ST69 Escherichia coli Strain in Italy