English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Nutrient limitation and nonfavorable growth conditions have been suggested to be major triggers for the expression of small, cysteine-rich antimicrobial proteins (AMPs) of fungal origin, e.g., the  Penicillium chrysogenum  antifungal protein (PAF), the  Aspergillus giganteus  antifungal protein (AFP), the  Aspergillus niger  antifungal protein (AnAFP). Therefore, these AMPs have been considered to be fungal secondary metabolite products. In contrast, the present study revealed that the expression of the PAF-related AMP  P. chrysogenum  antifungal protein B (PAFB) is strongly induced under nutrient excess during the logarithmic growth phase, whereas PAFB remained under the detection level in the supernatant of cultures grown under nutrient limitation. The efficiency of the  pafB -promoter to induce PAFB expression was compared with that of two  P. chrysogenum  promoters that are well established for recombinant protein production: the  paf -promoter and the xylose-inducible promoter of the xylanase gene,  xylP . The inducibility of the  pafB -promoter was superior to that of the  xylP -promoter yielding comparable PAFB amounts as under the regulation of the  paf -promoter. We conclude that (i) differences in the expression regulation of AMPs suggest distinct functional roles in the producer beyond their antifungal activity; and (ii) the  pafB -promoter is a promising tool for recombinant protein production in  P. chrysogenum , as it guarantees strong gene expression with the advantage of inducibility.

Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB