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Gram-positive actinomycete Rhodococcus jostii RHA1 is able to grow on C10 to C19 n -alkanes as a sole source of carbon and energy. To clarify, the n -alkane utilization pathway-a cluster of 5 genes ( alkBrubA1A2BalkU ) which appeared to be involved in n -alkane degradation-was identified and the transcriptional regulation of these genes was characterized. Reverse transcription-PCR analyses revealed that these genes constituted an operon and were transcribed in the presence of n -alkane. Inactivation of alkB led to the absence of the ability to utilize n -undecane. The alkB mutation resulted in reduction of growth rates on C10 and C12 n -alkanes; however, growths on C13 to C19 n -alkanes were not affected by this mutation. These results suggested that alkB was essential for the utilization of C10 to C12 n -alkanes. Inactivation of alkU showed the constitutive expression of alkB . Purified AlkU is able to bind to the putative promoter region of alkB , suggesting that AlkU played a role in repression of the transcription of alk operon. The results of this study indicated that alkB was involved in the medium-chain n -alkanes degradation of strain RHA1 and the transcription of alk operon was negatively regulated by alkU -encoded regulator. This report is important to understand the n -alkane degradation pathway of R. jostii , including the transcriptional regulation of alk gene cluster.

Characterization and Transcriptional Regulation of n-Alkane Hydroxylase Gene Cluster of Rhodococcus jostii RHA1