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Insufficient sensitivity is a general issue of colorimetric paper-based analytical devices (PADs) for trace analyte detection, such as metal ions, in environmental water. This paper demonstrates the colorimetric detection of zinc ions (Zn 2+ ) on a paper-based analytical device with an integrated analyte concentration system. Concentration of Zn 2+  ions from an enlarged sample volume (1 mL) has been achieved with the aid of a colorimetric Zn 2+  indicator (Zincon) electrostatically immobilized onto a filter paper substrate in combination with highly water-absorbent materials. Analyte concentration as well as sample pretreatment, including pH adjustment and interferent masking, has been elaborated. The resulting device enables colorimetric quantification of Zn 2+  in environmental water samples (tap water, river water) from a single sample application. The achieved detection limit of 0.53 μM is a significant improvement over that of a commercial colorimetric Zn 2+  test paper (9.7 μM), demonstrating the efficiency of the developed analyte concentration system not requiring any equipment.

Paper-Based Analytical Device for Zinc Ion Quantification in Water Samples with Power-Free Analyte Concentration