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We succeeded in simultaneously synthesizing the vitamin D family, vitamins D 2 , D 4 , D 5 , D 6 , and D 7 , from β-sitosterol, which is sold as a commercially available reagent from Tokyo Chemical Industry Co., Ltd. It is officially sold as a mixture of four phytosterols {β-sitosterol (40–45%), campesterol (20–30%), stigmasterol, and brassicasterol}. Owing to this, we anticipated that, using this reagent, various vitamin D analogs could be synthesized simultaneously. We also synthesized vitamin D 3  from pure cholesterol and analyzed and compared all vitamin D analogs (D 2 , D 3 , D 4 , D 5 , D 6 , and D 7 ) by HSQC NMR. We succeeded in clearly demonstrating the difference in the NMR chemical shifts for each vitamin D analog.

metabolites

Simultaneous Synthesis of Vitamins D2, D4, D5, D6, and D7 from Commercially Available Phytosterol, β-Sitosterol, and Identification of Each Vitamin D by HSQC NMR