English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

This study uses dynamic hyperpolarized [1- 13 C]pyruvate magnetic resonance spectroscopic imaging (MRSI) to estimate differences in glycolytic metabolism between highly metastatic (4T1,  n  = 7) and metastatically dormant (4T07,  n  = 7) murine breast cancer models. The apparent conversion rate of pyruvate-to-lactate (k PL ) and lactate-to-pyruvate area-under-the-curve ratio (AUC L/P ) were estimated from the metabolite images and compared with biochemical metabolic measures and immunohistochemistry (IHC). A non-significant trend of increasing k PL  ( p  = 0.17) and AUC L/P  ( p  = 0.11) from 4T07 to 4T1 tumors was observed. No significant differences in tumor IHC lactate dehydrogenase-A (LDHA), monocarboxylate transporter-1 (MCT1), cluster of differentiation 31 (CD31), and hypoxia inducible factor-α (HIF-1α), tumor lactate-dehydrogenase (LDH) activity, or blood lactate or glucose levels were found between the two tumor lines. However, AUC L/P  was significantly correlated with tumor LDH activity ( ρ  spearman  = 0.621,  p  = 0.027) and blood glucose levels ( ρ  spearman  = −0.474,  p  = 0.042). k PL  displayed a similar, non-significant trend for LDH activity ( ρ  spearman  = 0.480,  p  = 0.114) and blood glucose levels ( ρ  spearman  = −0.414,  p  = 0.088). Neither k PL  nor AUC L/P  were significantly correlated with blood lactate levels or tumor LDHA or MCT1. The significant positive correlation between AUC L/P  and tumor LDH activity indicates the potential of AUC L/P  as a biomarker of glycolytic metabolism in breast cancer models. However, the lack of a significant difference between in vivo tumor metabolism for the two models suggest similar pyruvate-to-lactate conversion despite differing metastatic potential.

Hyperpolarized 13C Magnetic Resonance Spectroscopic Imaging of Pyruvate Metabolism in Murine Breast Cancer Models of Different Metastatic Potential