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The effect of carbonic anhydrase (CA) on the separation performance of thin poly(amidoamine) (PAMAM) dendrimer/poly(ethylene glycol) (PEG) hybrid membranes was investigated. CA, a type of enzyme, was used to promote CO 2  hydration and dehydration reactions and to assess whether these reactions were the rate-limiting step in CO 2  permeation through the membrane. The relationship between the membrane thickness and the CO 2  permeance was evaluated in CO 2 /H 2  or CO 2 /He separation using PAMAM/PEG hybrid membranes (thickness: 10–100 μm) with and without CA. Without CA, the CO 2  permeance of PAMAM/PEG hybrid membranes was not inversely proportional to the membrane thickness. On the other hand, with CA, the CO 2  permeance was inversely proportional to the membrane thickness. It was implied that, without CA, the rate-limiting step of CO 2  transport was either the CO 2  hydration reaction at the feed side or the CO 2  dehydration reaction at the permeate side. On the other hand, with CA addition, the rate-limiting step of CO 2  transport was diffusion, and CO 2  permeance could be increased without sacrificing the selectivity by reducing membrane thickness. The effect of the position of CA (i.e., on the surface and/or reverse surface) on CO 2  separation performance was investigated to evaluate which reaction was the rate-limiting step of CO 2  permeation through the membrane. It was suggested that the rate-limiting step of CO 2  permeation was CO 2  dehydration reaction at the permeate side.

Effect of Carbonic Anhydrase on CO2 Separation Performance of Thin Poly(amidoamine) Dendrimer/Poly(ethylene glycol) Hybrid Membranes