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We developed a novel substrate made from fugitive glue (styrenic block copolymer) that can be used to analyze the effects of large strains on biological samples. The substrate has the following attributes: (1) It is easy to make from inexpensive components; (2) It is transparent and can be used in optical microscopy; (3) It is extremely stretchable as it can be stretched up to 700% strain; (4) It can be micro-molded, for example we created micro-ridges that are 6 μm high and 13 μm wide; (5) It is adhesive to biological fibers (we tested fibrin fibers), and can be used to uniformly stretch those fibers; (6) It is non-toxic to cells (we tested human mammary epithelial cells); (7) It can tolerate various salt concentrations up to 5 M NaCl and low (pH 0) and high (pH 14) pH values. Stretching of this extraordinary stretchable substrate is relatively uniform and thus, can be used to test multiple cells or fibers in parallel under the same conditions.

Highly Stretchable, Biocompatible, Striated Substrate Made from Fugitive Glue