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The fast-growing use of nanomaterials in everyday life raises the question about the safety of their use. Unfortunately, the risks associated with the use of nanoparticles (NPs) have not yet been fully assessed. The majority of studies conducted so far at the molecular and cellular level have focused on a single-type exposure, assuming that NPs act as the only factor. In the natural environment, however, we are likely exposed to a mixture of nanoparticles, whose interactions may modulate their impact on living organisms. This study aimed to evaluate the toxicological effects caused by in vitro exposure of HepG2 cells to AgNPs in combination with AuNPs, CdTe quantum dot (QD) NPs, TiO 2 NPs, or SiO 2 NPs. The results showed that the toxicity of nanoparticle binary mixtures depended on the type and ratio of NPs used. In general, the toxicity of binary mixtures of NPs was lower than the sum of toxicities of NPs alone (protective effect).

Susceptibility of HepG2 Cells to Silver Nanoparticles in Combination with other Metal/Metal Oxide Nanoparticles