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Cultural ecosystem services (ES) require a multidisciplinary approach. The aim of the study was to evaluate regional differences in the values of cultural ES in relation to natural capital in four small pilot regions of the Slovak Republic using a matrix system. The article is an approximation of the connection of the biophysical method of evaluation (matrix system) of cultural ES of natural capital with geospatial data at the regional level in the Slovak Republic. Within the natural potential of the ES cultural categories, we evaluated recreation and tourism, landscape character and aesthetics, natural and cultural heritage, knowledge base, and regional significance for the given region. The highest values of indices in all categories were found in the Brezno region. The results of the pilot regions of the Slovak Republic indicated that the terrain fragmentation in combination with a higher altitude and a larger area of forests and protected areas may represent significant factors influencing the potential of the area to provide the various benefits resulting from cultural ES. Even though there is significant monetary potential of cultural ES in the region, its intensive utilization is not readily apparent.

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Regional Differentiations of the Potential of Cultural Ecosystem Services in Relation to Natural Capital—A Case Study in Selected Regions of the Slovak Republic