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Satellite-derived lake surface water temperature (LSWT) measurements can be used for monitoring purposes. However, analyses based on the LSWT of Lake Ontario and the surrounding land surface temperature (LST) are scarce in the current literature. First, we provide an evaluation of the commonly used Moderate Resolution Imaging Spectroradiometer (MODIS)-derived LSWT/LST (MOD11A1 and MYD11A1) using in situ measurements near the area of where Lake Ontario, the St. Lawrence River and the Rideau Canal meet. The MODIS datasets agreed well with ground sites measurements from 2015–2017, with an R2 consistently over 0.90. Among the different ground measurement sites, the best results were achieved for Hill Island, with a correlation of 0.99 and centered root mean square difference (RMSD) of 0.73 K for Aqua/MYD nighttime. The validated MODIS datasets were used to analyze the temperature trend over the study area from 2001 to 2018, through a linear regression method with a Mann–Kendall test. A slight warming trend was found, with 95% confidence over the ground sites from 2003 to 2012 for the MYD11A1-Night datasets. The warming trend for the whole region, including both the lake and the land, was about 0.17 K year−1 for the MYD11A1 datasets during 2003–2012, whereas it was about 0.06 K year−1 during 2003–2018. There was also a spatial pattern of warming, but the trend for the lake region was not obviously different from that of the land region. For the monthly trends, the warming trends for September and October from 2013 to 2018 are much more apparent than those of other months.

A Slight Temperature Warming Trend Occurred over Lake Ontario from 2001 to 2018